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KMID : 0363620070280020213
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Journal of Korean Oriental Medicine
2007 Volume.28 No. 2 p.213 ~ p.223
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Study of Apoptotic Effect on Hydrogen Peroxide-induced Rat PC-12 Cells by Aster tataricus Water Extract
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Shin Yoo-Jung
Kim Seung-Mo
Park Chi-Sang
Shin Oh-Chul
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Abstract
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Objective : Alzheimer’s disease (AD) is a geriatric dementia that is widespread in old age. With an aging populace, AD is a looming problem in public health service. Alzheimer’s disease is characterized by specific neuronal degeneration in certain areas of the brain. Mutations and abnormal expression of several genes are associated with {beta}-amyloid deposits and Alzheimer’s disease; among them APP, PS1, and PS2, SOD, free radical, ROS.
Methods : We studied herbal medicines that have a relationship to brain degeneration. From pre-modern times, although a variety of oriental prescriptions of Aster tataricus have been traditionally utilized for the treatment of AD, their pharmacological effects and action mechanisms have not yet been fully elucidated.
Results : Based on morphological observations by phase-contrast microscope, TUNEL assay and MTT in the culture media, H_20_2-induced cell death was significantly inhibited by Aticus. We examined by ROS formation, catalase activity and GSH activity. We studied the protective effect and inhibitory effects of neurotoxicity in H_20_2-induced PC-12 cells by Aticus. Findings from our experiments show that Aticus inhibits apoptosis, which has neurotoxicities and cell damage in PC-12 cells. In addition, treatment with Aticus (>25{mu}g/ml for 6hrs) partially prevented H_20_2-induced cytotoxicity in PC-12 cells, and induced a protective effect.
Conclusion : As the result of this study, in the Aticus group, the apoptosis in the nervous system was inhibited, protected against the degeneration of PC-12 cells by H_20_2. Taken together, Aticus exhibited inhibition of H_20_2-induced apoptotic cell death. Aticus was found to induce protective effect on GSH and catalase in PC-12 cells. Based on these findings, Aticus may be beneficial for the treatment of AD.
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KEYWORD
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Alzheimer’s disease (AD), Aster tataricus, brain degeneration
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